OrangerV1, France, Analysis, bibRecord, 000153

Beta-cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model.

Identifieur interne : 000153 ( France/Analysis ); précédent : 000152; suivant : 000154

Beta-cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model.

Auteurs : Claudie Dhuique-Mayer [France] ; Patrick Borel ; Emmanuelle Reboul ; Bertrand Caporiccio ; Pierre Besancon ; Marie-Josèphe Amiot

Source :

The British journal of nutrition [ 0007-1145 ] ; 2007.

RBID : pubmed:17381979

English descriptors

KwdEn :
- Anticarcinogenic Agents (pharmacokinetics), Beverages, Biological Availability, Caco-2 Cells, Citrus (chemistry), Citrus sinensis, Cryptoxanthins, Digestion (physiology), Humans, Intestinal Absorption (physiology), Laurates (pharmacokinetics), Micelles, Models, Biological, Myristates (pharmacokinetics), Xanthophylls (pharmacokinetics), beta Carotene (pharmacokinetics).
MESH :
- chemical , pharmacokinetics : Anticarcinogenic Agents, Laurates, Myristates, Xanthophylls, beta Carotene.
- chemistry : Citrus.
- physiology : Digestion, Intestinal Absorption.
- Beverages, Biological Availability, Caco-2 Cells, Citrus sinensis, Cryptoxanthins, Humans, Micelles, Models, Biological.

Abstract

Beta-Cryptoxanthin (beta-CX), a provitaminic carotenoid of potential interest for health, is found principally in Citrus fruit in both free and esterified forms. Little is known about the intestinal absorption of beta-CX especially with regard to the esterified forms. The aim of this study was to evaluate the absorption of free and esterified beta-CX using simulated digestion coupled with the Caco-2 model. Bioaccessibility was investigated by measuring the transfer of carotenoids from different citrus juices into micelles using an in vitro digestion system. Then, carotenoid uptake was evaluated by adding carotenoid-rich micelles (from the in vitro digestion) or synthetic micelles (made from synthetic lipids and carotenoids purified from citrus juice) to human intestinal cells (Caco-2 TC7 clone). Our results showed that beta-cryptoxanthin esters (beta-CXE) were partially hydrolysed during the in vitro digestion. The bioaccessibility of free beta-CX measured was significantly higher (40 (SD 1.05) %) than that of beta-carotene (30 (SD 1.9) %) and beta-CXE (16 (SD 1.5) %). In the same way, the incorporation of free beta-CX (27 (SD 1.01) %) into synthetic micelles exceeded (P<0.05) that of beta-carotene (10 (SD 0.7) %) and beta-CXE (8.8 (SD 0.4) %). In the case of micelles from in vitro digestion, the uptake of beta-carotene, free beta-CX and beta-CXE forms by Caco-2 cells was 14.3 (SD 1.8), 3.9 (SD 1.3), and 0.7 (SD 0.08) % respectively. These results showed a preferential uptake by Caco-2 cells of beta-carotene and free beta-CX compared with the two esters of beta-CX.

DOI: 10.1017/S0007114507670822
PubMed: 17381979

Affiliations:

Links to Exploration step

pubmed:17381979

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Beta-cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model.</title>
<author><name sortKey="Dhuique Mayer, Claudie" sort="Dhuique Mayer, Claudie" uniqKey="Dhuique Mayer C" first="Claudie" last="Dhuique-Mayer">Claudie Dhuique-Mayer</name>
<affiliation wicri:level="3"><nlm:affiliation>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UR 24, Tropical food quality, TA50/04, 34398 Montpellier cedex 5, France. claudie.dhuique-mayer@cirad.fr</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UR 24, Tropical food quality, TA50/04, 34398 Montpellier cedex 5</wicri:regionArea>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Borel, Patrick" sort="Borel, Patrick" uniqKey="Borel P" first="Patrick" last="Borel">Patrick Borel</name>
</author>
<author><name sortKey="Reboul, Emmanuelle" sort="Reboul, Emmanuelle" uniqKey="Reboul E" first="Emmanuelle" last="Reboul">Emmanuelle Reboul</name>
</author>
<author><name sortKey="Caporiccio, Bertrand" sort="Caporiccio, Bertrand" uniqKey="Caporiccio B" first="Bertrand" last="Caporiccio">Bertrand Caporiccio</name>
</author>
<author><name sortKey="Besancon, Pierre" sort="Besancon, Pierre" uniqKey="Besancon P" first="Pierre" last="Besancon">Pierre Besancon</name>
</author>
<author><name sortKey="Amiot, Marie Josephe" sort="Amiot, Marie Josephe" uniqKey="Amiot M" first="Marie-Josèphe" last="Amiot">Marie-Josèphe Amiot</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2007">2007</date>
<idno type="RBID">pubmed:17381979</idno>
<idno type="pmid">17381979</idno>
<idno type="doi">10.1017/S0007114507670822</idno>
<idno type="wicri:Area/PubMed/Corpus">000B33</idno>
<idno type="wicri:Area/PubMed/Curation">000B33</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000B33</idno>
<idno type="wicri:Area/Ncbi/Merge">000687</idno>
<idno type="wicri:Area/Ncbi/Curation">000687</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000687</idno>
<idno type="wicri:doubleKey">0007-1145:2007:Dhuique Mayer C:beta:cryptoxanthin:from</idno>
<idno type="wicri:Area/Main/Merge">002415</idno>
<idno type="wicri:Area/Main/Curation">002335</idno>
<idno type="wicri:Area/Main/Exploration">002335</idno>
<idno type="wicri:Area/France/Extraction">000153</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Beta-cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model.</title>
<author><name sortKey="Dhuique Mayer, Claudie" sort="Dhuique Mayer, Claudie" uniqKey="Dhuique Mayer C" first="Claudie" last="Dhuique-Mayer">Claudie Dhuique-Mayer</name>
<affiliation wicri:level="3"><nlm:affiliation>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UR 24, Tropical food quality, TA50/04, 34398 Montpellier cedex 5, France. claudie.dhuique-mayer@cirad.fr</nlm:affiliation>
<country xml:lang="fr">France</country>
<wicri:regionArea>Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UR 24, Tropical food quality, TA50/04, 34398 Montpellier cedex 5</wicri:regionArea>
<wicri:noRegion>34398 Montpellier cedex 5</wicri:noRegion>
<placeName><region type="region" nuts="2">Occitanie (région administrative)</region>
<region type="old region" nuts="2">Languedoc-Roussillon</region>
<settlement type="city">Montpellier</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Borel, Patrick" sort="Borel, Patrick" uniqKey="Borel P" first="Patrick" last="Borel">Patrick Borel</name>
</author>
<author><name sortKey="Reboul, Emmanuelle" sort="Reboul, Emmanuelle" uniqKey="Reboul E" first="Emmanuelle" last="Reboul">Emmanuelle Reboul</name>
</author>
<author><name sortKey="Caporiccio, Bertrand" sort="Caporiccio, Bertrand" uniqKey="Caporiccio B" first="Bertrand" last="Caporiccio">Bertrand Caporiccio</name>
</author>
<author><name sortKey="Besancon, Pierre" sort="Besancon, Pierre" uniqKey="Besancon P" first="Pierre" last="Besancon">Pierre Besancon</name>
</author>
<author><name sortKey="Amiot, Marie Josephe" sort="Amiot, Marie Josephe" uniqKey="Amiot M" first="Marie-Josèphe" last="Amiot">Marie-Josèphe Amiot</name>
</author>
</analytic>
<series><title level="j">The British journal of nutrition</title>
<idno type="ISSN">0007-1145</idno>
<imprint><date when="2007" type="published">2007</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anticarcinogenic Agents (pharmacokinetics)</term>
<term>Beverages</term>
<term>Biological Availability</term>
<term>Caco-2 Cells</term>
<term>Citrus (chemistry)</term>
<term>Citrus sinensis</term>
<term>Cryptoxanthins</term>
<term>Digestion (physiology)</term>
<term>Humans</term>
<term>Intestinal Absorption (physiology)</term>
<term>Laurates (pharmacokinetics)</term>
<term>Micelles</term>
<term>Models, Biological</term>
<term>Myristates (pharmacokinetics)</term>
<term>Xanthophylls (pharmacokinetics)</term>
<term>beta Carotene (pharmacokinetics)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacokinetics" xml:lang="en"><term>Anticarcinogenic Agents</term>
<term>Laurates</term>
<term>Myristates</term>
<term>Xanthophylls</term>
<term>beta Carotene</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Digestion</term>
<term>Intestinal Absorption</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Beverages</term>
<term>Biological Availability</term>
<term>Caco-2 Cells</term>
<term>Citrus sinensis</term>
<term>Cryptoxanthins</term>
<term>Humans</term>
<term>Micelles</term>
<term>Models, Biological</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Beta-Cryptoxanthin (beta-CX), a provitaminic carotenoid of potential interest for health, is found principally in Citrus fruit in both free and esterified forms. Little is known about the intestinal absorption of beta-CX especially with regard to the esterified forms. The aim of this study was to evaluate the absorption of free and esterified beta-CX using simulated digestion coupled with the Caco-2 model. Bioaccessibility was investigated by measuring the transfer of carotenoids from different citrus juices into micelles using an in vitro digestion system. Then, carotenoid uptake was evaluated by adding carotenoid-rich micelles (from the in vitro digestion) or synthetic micelles (made from synthetic lipids and carotenoids purified from citrus juice) to human intestinal cells (Caco-2 TC7 clone). Our results showed that beta-cryptoxanthin esters (beta-CXE) were partially hydrolysed during the in vitro digestion. The bioaccessibility of free beta-CX measured was significantly higher (40 (SD 1.05) %) than that of beta-carotene (30 (SD 1.9) %) and beta-CXE (16 (SD 1.5) %). In the same way, the incorporation of free beta-CX (27 (SD 1.01) %) into synthetic micelles exceeded (P<0.05) that of beta-carotene (10 (SD 0.7) %) and beta-CXE (8.8 (SD 0.4) %). In the case of micelles from in vitro digestion, the uptake of beta-carotene, free beta-CX and beta-CXE forms by Caco-2 cells was 14.3 (SD 1.8), 3.9 (SD 1.3), and 0.7 (SD 0.08) % respectively. These results showed a preferential uptake by Caco-2 cells of beta-carotene and free beta-CX compared with the two esters of beta-CX.</div>
</front>
</TEI>
<affiliations><list><country><li>France</li>
</country>
<region><li>Languedoc-Roussillon</li>
<li>Occitanie (région administrative)</li>
</region>
<settlement><li>Montpellier</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Amiot, Marie Josephe" sort="Amiot, Marie Josephe" uniqKey="Amiot M" first="Marie-Josèphe" last="Amiot">Marie-Josèphe Amiot</name>
<name sortKey="Besancon, Pierre" sort="Besancon, Pierre" uniqKey="Besancon P" first="Pierre" last="Besancon">Pierre Besancon</name>
<name sortKey="Borel, Patrick" sort="Borel, Patrick" uniqKey="Borel P" first="Patrick" last="Borel">Patrick Borel</name>
<name sortKey="Caporiccio, Bertrand" sort="Caporiccio, Bertrand" uniqKey="Caporiccio B" first="Bertrand" last="Caporiccio">Bertrand Caporiccio</name>
<name sortKey="Reboul, Emmanuelle" sort="Reboul, Emmanuelle" uniqKey="Reboul E" first="Emmanuelle" last="Reboul">Emmanuelle Reboul</name>
</noCountry>
<country name="France"><region name="Occitanie (région administrative)"><name sortKey="Dhuique Mayer, Claudie" sort="Dhuique Mayer, Claudie" uniqKey="Dhuique Mayer C" first="Claudie" last="Dhuique-Mayer">Claudie Dhuique-Mayer</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/France/Analysis

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000153 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000153 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    France
   |étape=   Analysis
   |type=    RBID
   |clé=     pubmed:17381979
   |texte=   Beta-cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/France/Analysis/RBID.i   -Sk "pubmed:17381979" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/France/Analysis/biblio.hfd   \
       | NlmPubMed2Wicri -a OrangerV1

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

Serveur d'exploration sur l'oranger

Beta-cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model.

Beta-cryptoxanthin from citrus juices: assessment of bioaccessibility using an in vitro digestion/Caco-2 cell culture model.

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.